File:Scary faces of wood 4.tif
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[edit]DescriptionScary faces of wood 4.tif |
English: The scary faces of wood. The set of popper wood samples were treated with the catalyst (increasing incubation time in the set of images) in order to optimise the extraction of lignin polymers. Slices of 10 um thickness were cut with a microtome, mounted on slides, and imaged with a multicolour fluorescence microscope with laser excitation lines between 405 and 638 nm (four pseudo-coloured channels in the images). Temporal stacks of images (200 images/channel at 10 Hz) were generated with a 1.49 N.A. objective (Olympus) and an iXon Ultra 897 EMCCD camera (Andor) cooled to -100°C; and analysed with the Super-Resolution Radial Fluctuation (SRRF) algorithm. The summary magnification of the images was 600x (pixel size 26.7 nm). For the majority of the images, Fourier ring correlations (FRCs) indicates a minimum resolution of less than 60 nm (below the diffraction limit of optical microscopy). This allows the observation of individual lignin fibers in the cell walls. The change in intensity and colour across the series of images indicates the initial increase in the degree of conjugation in the polymer (the change from blue to red) with the subsequent extraction of products (the decrease in overall intensity and loss of cell wall stiffness). English: Super-resolution fluorescence microscopy image of popper wood cells (4-th image in set) |
Date | |
Source | Own work |
Author | Sergei Kopanchuk |
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[edit]I, the copyright holder of this work, hereby publish it under the following license:
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This file was uploaded as part of Wiki Science Competition 2021. |
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 13:13, 15 December 2021 | 1,280 × 1,280 (4.18 MB) | Skytal (talk | contribs) | Uploaded own work with UploadWizard |
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Width | 1,280 px |
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Height | 1,280 px |
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Compression scheme | LZW |
Pixel composition | RGB |
Number of components | 3 |
Number of rows per strip | 1 |
Horizontal resolution | 375,939.849 dpc |
Vertical resolution | 375,939.849 dpc |
Data arrangement | planar format |
Software used | OME Bio-Formats (unknown version) |