File:Prymnesium parvum Gr3 lrg.jpg
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Ploidy inference of closely related ''Prymnesium parvum'' strains ODER1 (left) and K-0081 (right)
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[edit]DescriptionPrymnesium parvum Gr3 lrg.jpg |
English: (A) As expected for a diploid organism, the Prymnesium parvom ODER1 strain showed a peak at 50 % allele frequency, while the K-0081 strain had a peak at 33% and a shoulder at around 60 %, suggesting a triploid rather than tetraploid genome. The number of homozygous variants in K-0081 was low (28,884 variants with 100 % allele frequency and coverage ≥20×), underlining the very close relationship of both strains. (B) Simultaneous flow cytometric analysis of the type B strains ODER1 and K-0081. The relative fluorescence of nuclei stained with propidium iodide shows ploidy-level difference between the strains. The resulting DNA content of strain ODER1 (0.55 pg) corresponds to diploidy, while the DNA content of strain K-0081 (0.77 pg) corresponds to triploidy. (C and D) Transmission electron microscopy (TEM) showing the body scales of the outer and inner layers of P. parvum (C) strain ODER1 and (D) strain K-0081. Scales of the outer layer are characterized by a narrow rim (black asterisk) and those of the inner layer by a wide rim (white asterisk). Scales of ODER1 exhibit a radial arrangement of microfibrils on both faces (typical for diploid cells) whereas scales of K-0081 exhibit a radial pattern on the proximal face (arrow) and a spirally wound pattern on the distal face (arrowhead). Scale bars, 400 nm. |
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Source | The haplotype-resolved Prymnesium parvum (type B) microalga genome reveals the genetic basis of its fish-killing toxins. In: Current Biology (2024). doi:10.1016/j.cub.2024.06.033 | |||
Author | Heiner Kuhl, Jürgen F. H. Strassert, Dora Čertnerová, Elisabeth Varga, Eva Kreuz, Dunja K. Lamatsch, Sven Wuertz, Jan Köhler, Michael T. Monaghan, Matthias Stöck | |||
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current | 12:53, 11 July 2024 | ![]() | 2,167 × 2,255 (1.01 MB) | Ernsts (talk | contribs) | Uploaded a work by Heiner Kuhl, Jürgen F. H. Strassert, Dora Čertnerová, Elisabeth Varga, Eva Kreuz, Dunja K. Lamatsch, Sven Wuertz, Jan Köhler, Michael T. Monaghan, Matthias Stöck from ''The haplotype-resolved ''Prymnesium parvum'' (type B) microalga genome reveals the genetic basis of its fish-killing toxins.'' In: ''Current Biology'' (2024). doi:10.1016/j.cub.2024.06.033 with UploadWizard |
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