File:MRA.00447-19-f0001-CruV-MCn-genomes.jpg

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English: (A) Outline of methodology used for detection and recovery of cruciviral sequences from environmental samples. Blue arrows represent the first steps after sample collection, and yellow arrows are the follow-up steps. envDNA, environmental DNA; MDA, multiple displacement amplification; iPCR, inverse PCR. (B) Genome structure of the cruciviruses found in Mill Creek, Woodburn, OR.
Object location45° 09′ 14.1″ N, 122° 50′ 40.1″ W Kartographer map based on OpenStreetMap.View this and other nearby images on: OpenStreetMapinfo
Putative capsid protein genes are depicted in green, putative Rep genes are in red, and putative origins of replication are in purple. A photograph of the sampled area is shown in the background. The location of Woodburn is indicated by a star on the map in the bottom-right corner. (C) Table indicating pairwise identity for the replication-associated protein (Rep) and the capsid protein (Cp) between each of the new cruciviral genomes. Sequences were aligned with MAFFT (L-INS-i; BLOSUM45; gap open penalty = 2.5; offset value = 0.123) in Geneious 11.0.4. The name, accession number, and percent identity of the best BLASTP hit for the new Cp and Rep sequences are indicated in the last column and row. Searches were performed on the NCBI Web server using the GenBank nonredundant protein sequence database on 29 April 2019. None of the top hits for Rep correspond to crucivirus sequences, while all of the Cp hits do.
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Source Fig. 1 at https://www.researchgate.net/publication/337150648_Genome_Sequences_of_Three_Cruciviruses_Found_in_the_Willamette_Valley_Oregon Genome Sequences of Three Cruciviruses Found in the Willamette Valley (Oregon). Microbiology Resource Announcements 8(23). e00447-19; doi:10.1128/MRA.00447-19
Author Ignacio de la Higuera, Ellis Lynn Torrance, Alyssa A. Pratt, George W. Kasun, Amberlee Maluenda, Kenneth M. Stedman
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current17:37, 26 March 2021Thumbnail for version as of 17:37, 26 March 2021711 × 1,281 (184 KB)Ernsts (talk | contribs)Uploaded a work by Ignacio de la Higuera, Ellis Lynn Torrance, Alyssa A. Pratt, George W. Kasun, Amberlee Maluenda, Kenneth M. Stedman from Fig. 1 at https://www.researchgate.net/publication/337150648_Genome_Sequences_of_Three_Cruciviruses_Found_in_the_Willamette_Valley_Oregon Genome Sequences of Three Cruciviruses Found in the Willamette Valley (Oregon). Microbiology Resource Announcements 8(23). e00447-19; doi:10.1128/MRA.00447-1950px with UploadWizard

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