File:Cell.2024.07.053.gr1 lrg.jpg

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Discovery of a pathogenic parvovirus (Zophobas morio black wasting virus, ZmBWV, currently unclassified ''Densovirinae'') via cryo-EM

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Description
English: Discovery of a pathogenic parvovirus (Zophobas morio black wasting virus, ZmBWV, currently unclassified Densovirinae) via cryo-EM
(A) Top: deceased superworms (Zophobas morio) typifying the blackening in Z. morio black wasting disease (ZmBWD).
Bottom: progression of ZmBWD in 4-week-old Z. morio larvae.
(B) Sucrose step gradient from blackened Z. morio larvae displaying two fractions (arrows) at the 20% and 30% sucrose interfaces, respectively, which appear blue in fluorescent light due to the presence of viral particles.
(C) Electron micrographs displaying the particles of ∼28 nm from those fractions. Insets emphasize the absence of genome inside particles of the 20 % band and presence inside particles of the 30 % band.
(D) Isosurface rendering (left) and central slice through (right) 3D reconstructions of the particles purified from diseased larvae. The gold line separates reconstructions of the 20 % band (upper right) and 30 % band (lower left). The protein structure is almost identical between the two bands, but only the 30 % band contains genome. The initial polyalanine chain is displayed as a ribbon diagram, with each T = 1-related subunit in a different color.
(E) Comparison of the capsid surface of the densovirus described herein associated with ZmBWD and consequently designated as Zophobas morio black wasting virus (ZmBWV) with those of other members of the Parvoviridae family: subfamilies
  • Densovirinae (Galleria mellonella densovirus [GmDV], Acheta domesticus densovirus [AdDV], and Bombyx mori densovirus [BmDV]),
  • Hamaparvovirinae (Penaeus stylirostris densovirus [PstDV]), and
  • Parvovirinae (adeno-associated virus 2 [AAV2]),
colored by radial distance.
(F) Discovery of an Iflavirus (Iflaviridae) at low concentration alongside NJ2-molitor.
Left: extract of a micrograph showing an Iflavirus virion (arrow) alongside a ZmBWV virion.
Right: automated model of the reconstructed density, used for structure-based identification.
Date
Source Cryo-EM-based discovery of a pathogenic parvovirus causing epidemic mortality by black wasting disease in farmed beetles. In: Cell, August 28, 2024; doi:10.1016/j.cell.2024.07.053
Author Judit J. Penzes, Martin Holm, Samantha A. Yost, Jason T. Kaelber
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