File:Widefield-Two-Photon-Excitation-without-Scanning-Live-Cell-Microscopy-with-High-Time-Resolution-and-pone.0147115.s004.ogv

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Widefield-Two-Photon-Excitation-without-Scanning-Live-Cell-Microscopy-with-High-Time-Resolution-and-pone.0147115.s004.ogv ‎(Ogg Theora video file, length 12 s, 656 × 278 pixels, 181 kbps, file size: 263 KB)

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English: Reduced photo-bleaching using widefield two-photon excitation. Time-lapse recordings of live neuronal cells loaded with Fluo-4 AM at a frame rate of 100 Hz using single-photon (left) and two-photon (right) widefield excitation. The neuronal cultures were irradiated continuously for 590 seconds and the video shows a frame every 5 seconds, demonstrating very clearly the rapid decrease in fluorescence intensity observed using widefield single-photon excitation, while no significant reduction in fluorescence intensity was observed using widefield two-photon excitation. Scale bar = 15 μm.
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Source S1 Video from Amor R, McDonald A, Trägårdh J, Robb G, Wilson L, Abdul Rahman N, Dempster J, Amos W, Bushell T, McConnell G (2016). "Widefield Two-Photon Excitation without Scanning: Live Cell Microscopy with High Time Resolution and Low Photo-Bleaching". PLOS ONE. DOI:10.1371/journal.pone.0147115. PMID 26824845. PMC: 4732674.
Author Amor R, McDonald A, Trägårdh J, Robb G, Wilson L, Abdul Rahman N, Dempster J, Amos W, Bushell T, McConnell G
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current10:49, 7 February 201612 s, 656 × 278 (263 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 360P 61 kbps Completed 04:39, 27 October 2018 3.0 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 29 kbps Completed 04:39, 27 October 2018 2.0 s
Streaming 240p (VP9) 29 kbps Completed 12:10, 5 December 2023 1.0 s
WebM 360P 424 kbps Completed 10:49, 7 February 2016 4.0 s
Streaming 144p (MJPEG) 325 kbps Completed 23:38, 18 November 2023 1.0 s

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