File:Two-Photon-Microscopy-Imaging-of-thy1GFP-M-Transgenic-Mice-A-Novel-Animal-Model-to-Investigate-pone.0056144.s006.ogv

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Original file ‎(Ogg Theora video file, length 6.0 s, 900 × 699 pixels, 285 kbps, file size: 209 KB)

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English: Motile non-neuronal GFP+ cells observed through the thinned skull surgery. In vivo TPF microscopy movie of non-neuronal GFP+ cells in a thy1GFP-M mouse in which the skull was thinned out (see section 2.2). GFP+ cells show the same motility observed in the open skull preparation, indicating that surgery did not affect the behavior of these cells. On the left, a GFP+ cell in a probing motion; in the center, a GFP+ cell in a scanning motion on the cortical surface; at minute 10, a GFP+ cell appears for one only frame and is probably floating in the cerebrospinal fluid. Each frame is a maximum intensity projections of a set of optical sections acquired at 2.5 µm z-step. Image depth: 73 µm from dura mater. Blood vasculature is visualized by iv injected tetramethyl rhodamine isothiocyanate-conjugated dextran.
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Source Movie S4 from Laperchia C, Allegra Mascaro A, Sacconi L, Andrioli A, Matte A, De Franceschi L, Grassi-Zucconi G, Bentivoglio M, Buffelli M, Pavone F (2013). "Two-Photon Microscopy Imaging of thy1GFP-M Transgenic Mice: A Novel Animal Model to Investigate Brain Dendritic Cell Subsets In Vivo". PLOS ONE. DOI:10.1371/journal.pone.0056144. PMID 23409142. PMC: 3567047.
Author Laperchia C, Allegra Mascaro A, Sacconi L, Andrioli A, Matte A, De Franceschi L, Grassi-Zucconi G, Bentivoglio M, Buffelli M, Pavone F
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