File:Trypanosome-Motion-Represents-an-Adaptation-to-the-Crowded-Environment-of-the-Vertebrate-Bloodstream-ppat.1003023.s010.ogv

English: Efficient locomotion of trypanosomes in PDMS pillar arrays. (A, B) Trypanosomes were fluorescently surface-labeled with AMCA-sulfo-NHS and observed in regular 4 µm-PDMS pillar arrays. High-speed videos were recorded using sCMOS technology (pco.edge camera). (C) Transmitted light microscopic example of trypanosomes swimming in arrays of pillars with two different sizes (10 and 12 µm). (D) Tracing fluorescently labeled trypanosomes in pillar arrays shows maximum swimming velocities of about 40 µm s−1. (E) Maximum intensity projection (left) and selected still images from a high-speed fluorescence (xyt) image series of a fluorescently labeled trypanosome swimming through an array of pillars (4 µm spacing), acquired at a frame-rate of 400 images per second. Note that the curvatures of the flagellum and the cell body closely reflect the shape of the pillars (positions indicated by “x”). Scale bar = 5 µm. (F) Illustration of the experimental setup.