File:Trypanosome-Motion-Represents-an-Adaptation-to-the-Crowded-Environment-of-the-Vertebrate-Bloodstream-ppat.1003023.s003.ogv

English: (A) The path of the flagellum around the trypanosome cell body in three-dimensional representations. Trypanosomes were fluorescently surface-labeled with AMCA-sulfo-NHS, fixed and recorded as wide-field xyz stacks. The original and deconvolved stacks are shown. (B) The 3 d volume models generated from the deconvolved stacks are animated in a 180° turn and show the pathway of the flagellum wrapped approximately a half turn around the cell body. Using the volume models, the complete flagellum was traced and measured in three-dimensional space. The 180° turn relative to the body was registered on average along the first 11 µm of the flagellum, as measured from the emergence point at the posterior flagellar pocket, defined as the first anchor point, to the point on the opposite side of the cell body where the flagellum stopped wrapping around the body surface, defined as the second anchor point. The second third of the flagellum's length (full length ∼30 µm) followed the convex surface of the cell body. For clarity, the last free third of the flagellum is only shown for one cell.