File:Transient-Neuronal-Populations-Are-Required-to-Guide-Callosal-Axons-A-Role-for-Semaphorin-3C-pbio.1000230.s009.ogv

English: The neurons of the CC cooperate to form complex neuronal network. Video S1 was coded using DivX codec. The DivX coded for Windows can be downloaded free on the following site: http://www.divx.com/divx/windows/. Downloading this allows the viewing of video S1. Downloading video S1 can take around 30 s. The geometry of the cell-free spaces and the cellular environment of the isosurface for the three stains (CR, Mash1-GFP, and Hoechst) are explored with the navigator function of IMARIS 4.3 software. The 3-D reconstruction is reoriented according to the direction of axonal growth within the CC. Exploring the thickness of the 3-D stack imaging of the CC reveals that the CR-positive neurons (red) and the GFP-positive GABAergic interneurons (green) form adjacent rows of neurons regularly distributed within the entire CC. Rows of neurons are separated by cell-free domains of small or large diameter, which likely segregate the callosal axons into bundles. This network of cell-free domain could define preferred pathways for the navigation of callosal axons. This analysis shows that the two types of neurons that transiently populate the CC form a complex neuronal network and are well positioned to interact with ingrowing callosal axons.