File:Synthetic-Protocells-Interact-with-Viral-Nanomachinery-and-Inactivate-Pathogenic-Human-Virus-pone.0016874.s002.ogv

English: Movie showing rotation of three dimensional image based on three dimensional deconvolution of fluorescent confocal z-stack images. The experiment consisted of engineered HeLa cells expressing HeV G and F on their membrane interacting with EFNB2 bearing protocells to evaluate the bilayer formation on the protocells and protocell - viral binding moiety interactions. The video shows well formed lipid layers wrapped around each protocell and also shows the protocells binding to cell membranes. In this reduced system, like the experiments with soluble G binding to protocells and analyzed with flow cytometry, simple binding does occur between EFNB2 bearing protocells and cells membranes' expressing HeV G and F. However, there is neither internalization of the protocells nor fusion of the protocells and cell membranes. The HeLa cells were expressing green fluorescent protein in the cytoplasm for imaging; the protocells were synthesized with red fluorescent lipid for imaging.