File:Stage-Specific-Changes-in-the-Water-Na+-Cl--and-K+-Contents-of-Organelles-during-Apoptosis-pone.0148727.s009.ogv
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Stage-Specific-Changes-in-the-Water-Na+-Cl--and-K+-Contents-of-Organelles-during-Apoptosis-pone.0148727.s009.ogv (Ogg Theora video file, length 3.3 s, 472 × 456 pixels, 19.95 Mbps, file size: 7.93 MB)
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[edit]DescriptionStage-Specific-Changes-in-the-Water-Na+-Cl--and-K+-Contents-of-Organelles-during-Apoptosis-pone.0148727.s009.ogv |
English: Simultaneous 3D localization of cytochrome- c (Cc) and H2B-GFP showing Cc redistribution during specific stages of apoptosis. Anti-cytochrome-c antibody binding was imaged on fixed HeLa cells stably expressing H2B-GFP after the induction of apoptosis by 500 ng/mL AMD, for 7h and 15 minutes. For each stage of apoptosis and stage 0, Z-stacks were processed for a simultaneous 3D transparent volume rendering of Cc (red) and H2B-GFP (green) which was then fully rotated. The six cells (ST1 to ST5 and ST0) are shown in the same window to improve comparison of Cc and H2B-GFP localization. |
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Source | S6 Movie from Nolin F, Michel J, Wortham L, Tchelidze P, Banchet V, Lalun N, Terryn C, Ploton D (2016). "Stage-Specific Changes in the Water, Na+, Cl- and K+ Contents of Organelles during Apoptosis, Demonstrated by a Targeted Cryo Correlative Analytical Approach". PLOS ONE. DOI:10.1371/journal.pone.0148727. PMID 26866363. PMC: 4807926. | ||
Author | Nolin F, Michel J, Wortham L, Tchelidze P, Banchet V, Lalun N, Terryn C, Ploton D | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 13:55, 13 October 2016 | 3.3 s, 472 × 456 (7.93 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Simultaneous 3D localization of cytochrome- c (Cc) and H2B-GFP showing Cc redistribution during specific stages of apoptosis. |
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Author | Nolin F, Michel J, Wortham L, Tchelidze P, Banchet V, Lalun N, Terryn C, Ploton D |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Anti-cytochrome-c antibody binding was imaged on fixed HeLa cells stably expressing H2B-GFP after the induction of apoptosis by 500 ng/mL AMD, for 7h and 15 minutes. For each stage of apoptosis and stage 0, Z-stacks were processed for a simultaneous 3D transparent volume rendering of Cc (red) and H2B-GFP (green) which was then fully rotated. The six cells (ST1 to ST5 and ST0) are shown in the same window to improve comparison of Cc and H2B-GFP localization. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-02-11 |