File:Selective-Infection-of-Antigen-Specific-B-Lymphocytes-by-Salmonella-Mediates-Bacterial-Survival-and-pone.0050667.s006.ogv

English: GFP-Salmonella is excreted from primary human B cells. Living primary B cells infected with GFP-expressing Salmonella and co-cultured with CD40L-expressing 3T3 cells were imaged using widefield fluorescence microscopy at 37°C. Depicted are GFP and Texas-Red signals projected on the transmission image with images taken every 30 min. A single primary B cell that has phagocytosed anti-BCR coated GFP-expressing Salmonella is followed over time in medium containing anti-LPS antibodies labeled with Texas-Red. The bacterium is protected from staining by the extracellular antibodies as long as it resides intracellular. Double labeling is observed after 6.5 hr, showing access of the B cell-associated Salmonella to the antibody-containing medium. Total duration: 11.5 hours.