File:Real-time-tracking-of-cell-cycle-progression-during-CD8+-effector-and-memory-T-cell-differentiation-ncomms7301-s3.ogv

English: Supplementary Movie 2 Sequential divisions of smaller or larger sized T cells. Cell Trace Violet-labeled Fucci-CD8+ T cells were stimulated with plate-bound anti-CD3ε (1.0 μg/ml) and anti-CD28 mAb (0.5 μg/ml) and sorted to collect the cells in the 8th division generation. Sorted cells were seeded into microgrid arrays in the presence of rIL-2 (10 ng/ml) and subjected to time-lapse imaging every 3-4 min with 6-8 stacks at 0.8-1.2 μm (z-plane). Representative movie with smaller and larger size cells from 3rd day of imaging of sorted 8th generation. Movie duration: 3.5 hr, Objectives: 20× 0.5 NA.