File:Rac1-Dependent-Collective-Cell-Migration-Is-Required-for-Specification-of-the-Anterior-Posterior-pbio.1000442.s021.ogv

English: Lack of projections in a Rac1 null embryo. The embryo depicted in Video S9 is from a litter dissected on e5.5 at mid-afternoon and was selected because the Hex-GFP cells appeared to be round and to not have initiated migration. The embryo was smaller than its wild-type littermates at dissection but developed overnight to a degree similar to what we have observed in vivo. It was imaged from 6 pm to 10:30 am. Frames are 10 min apart. A stack of 23 slices was taken, at 2 µm per slice. The movie represents the first 5 h of imaging. It was genotyped the following day and confirmed to be Rac1 null.