File:Premitotic-Assembly-of-Human-CENPs--T-and--W-Switches-Centromeric-Chromatin-to-a-Mitotic-State-pbio.1001082.s010.ogv

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Premitotic-Assembly-of-Human-CENPs--T-and--W-Switches-Centromeric-Chromatin-to-a-Mitotic-State-pbio.1001082.s010.ogv ‎(Ogg Theora video file, length 10 s, 508 × 508 pixels, 604 kbps, file size: 747 KB)

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English: Tubulin labeling confirms whole spindle motion in CENP-W depleted cells. HeLa-H2B-GFP cells were transfected with mCherry-tubulin to directly visualize spindle motion following transfection with pooled siRNAs against CENP-W. It is clear that the entire spindle is moving within cells that exhibit rolling. The spindle poles can be seen to split in some of these cells, as though the forces associated with spindle movement are able to disrupt spindle pole integrity. This may account for the multipolar spindles frequently observed in fixed specimens of CENP-W siRNA-treated cells.
Date
Source Video S4 from Prendergast L, van Vuuren C, Kaczmarczyk A, Doering V, Hellwig D, Quinn N, Hoischen C, Diekmann S, Sullivan K (2011). "Premitotic Assembly of Human CENPs -T and -W Switches Centromeric Chromatin to a Mitotic State". PLOS Biology. DOI:10.1371/journal.pbio.1001082. PMID 21695110. PMC: 3114758.
Author Prendergast L, van Vuuren C, Kaczmarczyk A, Doering V, Hellwig D, Quinn N, Hoischen C, Diekmann S, Sullivan K
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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Date/TimeThumbnailDimensionsUserComment
current22:26, 14 November 201210 s, 508 × 508 (747 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 241 kbps Completed 09:16, 14 October 2018 6.0 s
Streaming 480p (VP9) Not ready Unknown status
VP9 360P 137 kbps Completed 09:16, 14 October 2018 5.0 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 83 kbps Completed 09:16, 14 October 2018 4.0 s
Streaming 240p (VP9) 84 kbps Completed 14:35, 6 December 2023 1.0 s
WebM 360P 515 kbps Completed 16:21, 15 November 2012 7.0 s
Streaming 144p (MJPEG) 454 kbps Completed 17:37, 19 November 2023 0.0 s

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