File:Photoswitching-Free-FRAP-Analysis-with-a-Genetically-Encoded-Fluorescent-Tag-pone.0107730.s002.ogv
Photoswitching-Free-FRAP-Analysis-with-a-Genetically-Encoded-Fluorescent-Tag-pone.0107730.s002.ogv (Ogg Theora video file, length 24 s, 300 × 300 pixels, 5.12 Mbps, file size: 14.65 MB)
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DescriptionPhotoswitching-Free-FRAP-Analysis-with-a-Genetically-Encoded-Fluorescent-Tag-pone.0107730.s002.ogv |
English: A representative movie of a circular spot FRAP of histone H2B. A spot of 1.5 µm in diameter in a nucleus expressing H2B-Halo-TMR (surrounded by a yellow circle) was photobleached at 4 sec after starting the image acquisition. The 4 sec of pre-bleach and the 20 sec of post-bleach images are shown at the same frame rate (30 Hz). There is a very small fast recovery which is followed by a very slow recovery. The corresponding FRAP curve is shown in Fig. 2C and Fig. S6 as red curves. |
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Source | Movie S2 from Morisaki T, McNally J (2014). "Photoswitching-Free FRAP Analysis with a Genetically Encoded Fluorescent Tag". PLOS ONE. DOI:10.1371/journal.pone.0107730. PMID 25233348. PMC: 4169462. | |||
Author | Morisaki T, McNally J | |||
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current | 22:51, 10 December 2014 | 24 s, 300 × 300 (14.65 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Morisaki T, McNally J |
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Usage terms | http://creativecommons.org/publicdomain/zero/1.0/ |
Image title | A representative movie of a circular spot FRAP of histone H2B. A spot of 1.5 µm in diameter in a nucleus expressing H2B-Halo-TMR (surrounded by a yellow circle) was photobleached at 4 sec after starting the image acquisition. The 4 sec of pre-bleach and the 20 sec of post-bleach images are shown at the same frame rate (30 Hz). There is a very small fast recovery which is followed by a very slow recovery. The corresponding FRAP curve is shown in Fig. 2C and Fig. S6 as red curves. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014 |