File:P12-Tethers-the-Murine-Leukemia-Virus-Pre-integration-Complex-to-Mitotic-Chromosomes-ppat.1003103.s007.ogv

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P12-Tethers-the-Murine-Leukemia-Virus-Pre-integration-Complex-to-Mitotic-Chromosomes-ppat.1003103.s007.ogv ‎(Ogg Theora video file, length 43 s, 480 × 360 pixels, 144 kbps, file size: 756 KB)

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English: PICs with mutant p12 proteins fail to dock to mitotic chromosomes. Interphase (A) and mitotic (B) cells of unsynchronized, PM14 GFP-infected U/R/RFP-H2A culture were visualized by time-lapse microscopy 12 hpi. The same procedure was applied to visualize mitotic cells of unsynchronized, PM14-infected NIH3T3/RFP-H2A culture (C); 2ME2-arrested U/R cells, co-infected with wt mCherry and PM14 GFP (D); and unsynchronized U/R/RFP-H2A cells infected with S(61, 65)A GFP (E) or with S(61, 65)A/M63I GFP (F). Broken lined circle marks chromosomal region with moving PM14 PICs with no apparent docking (B). Arrowheads point to representative wt mCherry PICs with stable chromosomal docking (D), or to an S(61, 65)A GFP PIC showing only transient, unstable association with the chromosomes (E). Frequency: 0.4, 0.4, 1.2, 0.8, 1.2 and 1.2 fps for A to F, respectively. Scale bar, 10 µm.
Date
Source Movie S5 from Elis E, Ehrlich M, Prizan-Ravid A, Laham-Karam N, Bacharach E (2012). "p12 Tethers the Murine Leukemia Virus Pre-integration Complex to Mitotic Chromosomes". PLOS Pathogens. DOI:10.1371/journal.ppat.1003103. PMID 23300449. PMC: 3531515.
Author Elis E, Ehrlich M, Prizan-Ravid A, Laham-Karam N, Bacharach E
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Date/TimeThumbnailDimensionsUserComment
current19:16, 21 January 201343 s, 480 × 360 (756 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 360P 76 kbps Completed 09:07, 6 September 2018 12 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 45 kbps Completed 09:07, 6 September 2018 11 s
Streaming 240p (VP9) 45 kbps Completed 23:05, 4 December 2023 1.0 s
WebM 360P 299 kbps Completed 19:17, 21 January 2013 16 s
Streaming 144p (MJPEG) 559 kbps Completed 18:59, 17 November 2023 2.0 s

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