File:Optogenetic-Control-of-PIP3-PIP3-Is-Sufficient-to-Induce-the-Actin-Based-Active-Part-of-Growth-pone.0070861.s011.ogv
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Optogenetic-Control-of-PIP3-PIP3-Is-Sufficient-to-Induce-the-Actin-Based-Active-Part-of-Growth-pone.0070861.s011.ogv (Ogg Theora video file, length 6.1 s, 252 × 304 pixels, 599 kbps, file size: 444 KB)
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[edit]DescriptionOptogenetic-Control-of-PIP3-PIP3-Is-Sufficient-to-Induce-the-Actin-Based-Active-Part-of-Growth-pone.0070861.s011.ogv |
English: PIP3 elevation increased the number of Rab5a-positive endosomes. Time-lapse images of a growth cone of a stage 2 mouse hippocampal neuron expressing mCh-Rab5a and mVenus-PHR-iSH-2A-CIBNcaax. mCh-Rab5a fluorescence images were acquired every 10 s. The red circled area was continuously photoactivated (405-nm laser at 10% of maximal power, 0.1 Hz) from 0 min. Accumulation of Rab5a-positive vesicles increased in the C domain of the growth cone and in neurites in response to photoactivation. |
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Source | Movie S9 from Kakumoto T, Nakata T (2013). "Optogenetic Control of PIP3: PIP3 Is Sufficient to Induce the Actin-Based Active Part of Growth Cones and Is Regulated via Endocytosis". PLOS ONE. DOI:10.1371/journal.pone.0070861. PMC: 3737352. | ||
Author | Kakumoto T, Nakata T | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 06:01, 18 August 2013 | 6.1 s, 252 × 304 (444 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Kakumoto T, Nakata T |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | PIP3 elevation increased the number of Rab5a-positive endosomes. Time-lapse images of a growth cone of a stage 2 mouse hippocampal neuron expressing mCh-Rab5a and mVenus-PHR-iSH-2A-CIBNcaax. mCh-Rab5a fluorescence images were acquired every 10 s. The red circled area was continuously photoactivated (405-nm laser at 10% of maximal power, 0.1 Hz) from 0 min. Accumulation of Rab5a-positive vesicles increased in the C domain of the growth cone and in neurites in response to photoactivation. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |
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Categories:
- Cell motility
- Microfilaments
- Molecular cell biology
- Signal transduction
- Signaling cascades
- Polyphosphoinositide signaling cascade
- Signaling pathways
- Phosphoinositide signal transduction
- Cellular neuroscience
- Neuronal morphology
- Molecular neuroscience
- Synthetic biology
- Media from PLOS ONE
- Optogenetics
- Videos of laser-induced fluorescence