File:Non-invasive-in-vivo-investigation-of-hepatobiliary-structure-and-function-in-STII-medaka-(Oryzias-1476-5926-7-7-S5.ogv

English: Example of 3D reconstruction of parenchyma from in vivo confocal image stacks: relationship of sinusoids to intrahepatic biliary passageways, STII medaka, 30 dpf. Sinusoids (S) are red, canaliculi (C) in green. All space between sinusoids and surrounding canaliculi (empty) is hepatocellular space, not rendered for visual clarity. Morphometric and volumetric analyses of 3D reconstructions assisted elucidation of parenchymal architecture, and relationship of canaliculi to sinusoids. These types of investigations revealed medaka hepatic parenchyma to be more akin to a muralium like structure (as opposed to tubular architecture). Grayscale confocal image can be seen in the background. The movie given here, extracted from an Amira 3D reconstruction, is limited to rotation in a single plane. Actual 3D reconstructions can be rotated in any plane, at virtually any magnification, allowing detailed study of hepatobiliary structure/function relationships.