File:Neurons-are-the-Primary-Target-Cell-for-the-Brain-Tropic-Intracellular-Parasite-Toxoplasma-gondii-ppat.1005447.s007.ogv
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[edit]DescriptionNeurons-are-the-Primary-Target-Cell-for-the-Brain-Tropic-Intracellular-Parasite-Toxoplasma-gondii-ppat.1005447.s007.ogv |
English: 3-D rendering of Toxoplasma cyst located inside cell body. Imaris 7.6.5 software was used to create a 3-D movie from z-stack images through the II-Cre infected neuron in Fig 5a. After detection of the neuron cell body, neuronal processes and the cyst, a surface rendering is created and the background is removed. Full rotation of the z-stack reveals that the entire cyst is located within the cell body. Areas of the cell have been stretched so thin that the Imaris software is unable to detect the limited concentration of GFP in these areas, which creates a window-like appearance. On individual z-stack images, GFP can be found fully surrounding the cyst (S6 Fig). |
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Source | S1 Video from Cabral C, Tuladhar S, Dietrich H, Nguyen E, MacDonald W, Trivedi T, Devineni A, Koshy A (2016). "Neurons are the Primary Target Cell for the Brain-Tropic Intracellular Parasite Toxoplasma gondii". PLOS Pathogens. DOI:10.1371/journal.ppat.1005447. PMID 26895155. PMC: 4760770. | ||
Author | Cabral C, Tuladhar S, Dietrich H, Nguyen E, MacDonald W, Trivedi T, Devineni A, Koshy A | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 23:20, 8 March 2016 | 10 s, 1,536 × 878 (4.12 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | 3-D rendering of Toxoplasma cyst located inside cell body. |
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Author | Cabral C, Tuladhar S, Dietrich H, Nguyen E, MacDonald W, Trivedi T, Devineni A, Koshy A |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Imaris 7.6.5 software was used to create a 3-D movie from z-stack images through the II-Cre infected neuron in Fig 5a. After detection of the neuron cell body, neuronal processes and the cyst, a surface rendering is created and the background is removed. Full rotation of the z-stack reveals that the entire cyst is located within the cell body. Areas of the cell have been stretched so thin that the Imaris software is unable to detect the limited concentration of GFP in these areas, which creates a window-like appearance. On individual z-stack images, GFP can be found fully surrounding the cyst (S6 Fig). |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-02-19 |