File:Multicellular-Bacteria-Deploy-the-Type-VI-Secretion-System-to-Preemptively-Strike-Neighboring-Cells-ppat.1003608.s014.ogv
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Multicellular-Bacteria-Deploy-the-Type-VI-Secretion-System-to-Preemptively-Strike-Neighboring-Cells-ppat.1003608.s014.ogv (Ogg Theora video file, length 23 s, 480 × 360 pixels, 55 kbps, file size: 154 KB)
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[edit]DescriptionMulticellular-Bacteria-Deploy-the-Type-VI-Secretion-System-to-Preemptively-Strike-Neighboring-Cells-ppat.1003608.s014.ogv |
English: Visualization of P. mirabilis T6SS-activity within an opposing 9C1 mutant swarm. Strain HI4320 expressing VipA::sfGFP under control of pBAD is observed during infiltration deep within the 9C1 swarm. The appearance and disappearance of distinct puncta of green fluorescence indicates active and rapid assembly and firing of the T6SS during attack on the susceptible 9C1 cells. Swarms were visualized on swarm agar containing 10 mM L-arabinose to induce expression of the VipA::sfGFP translational fusion. The FITC (green) fluorescence is presented alone to maximize visualization of T6SS activity. |
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Source | Movie S5 from Alteri C, Himpsl S, Pickens S, Lindner J, Zora J, Miller J, Arno P, Straight S, Mobley H (2013). "Multicellular Bacteria Deploy the Type VI Secretion System to Preemptively Strike Neighboring Cells". PLOS Pathogens. DOI:10.1371/journal.ppat.1003608. PMC: 3764213. | ||
Author | Alteri C, Himpsl S, Pickens S, Lindner J, Zora J, Miller J, Arno P, Straight S, Mobley H | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 20:30, 14 September 2013 | 23 s, 480 × 360 (154 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Alteri C, Himpsl S, Pickens S, Lindner J, Zora J, Miller J, Arno P, Straight S, Mobley H |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Visualization of P. mirabilis T6SS-activity within an opposing 9C1 mutant swarm. Strain HI4320 expressing VipA::sfGFP under control of pBAD is observed during infiltration deep within the 9C1 swarm. The appearance and disappearance of distinct puncta of green fluorescence indicates active and rapid assembly and firing of the T6SS during attack on the susceptible 9C1 cells. Swarms were visualized on swarm agar containing 10 mM L-arabinose to induce expression of the VipA::sfGFP translational fusion. The FITC (green) fluorescence is presented alone to maximize visualization of T6SS activity. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013-09 |