File:Mitigating-Phototoxicity-during-Multiphoton-Microscopy-of-Live-Drosophila-Embryos-in-the-1.0-1.2-µm-pone.0104250.s003.ogv

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English: 3D imaging of an unstained Drosophila embryo with THG during gastrulation (stages 5–8). Imaging conditions: 57 s per 3D stack, 2 min between successive stacks, 750 nm per pixel lateral sampling, 2 µm per pixel axial sampling, 1180 nm excitation wavelength, 100 fs pulses. 3D THG imaging allows the monitoring of the ventral furrow formation. Scale bar  = 100 µm. See also figure 5.
Date
Source Video S3 from Debarre D, Olivier N, Supatto W, Beaurepaire E (2014). "Mitigating Phototoxicity during Multiphoton Microscopy of Live Drosophila Embryos in the 1.0-1.2 µm Wavelength Range". PLOS ONE. DOI:10.1371/journal.pone.0104250. PMID 25111506. PMC: 4128758.
Author Debarre D, Olivier N, Supatto W, Beaurepaire E
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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current02:31, 15 August 201423 s, 960 × 640 (3.81 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 480P 325 kbps Completed 13:58, 3 September 2018 27 s
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