File:Metabolomics-Driven-Nutraceutical-Evaluation-of-Diverse-Green-Tea-Cultivars-pone.0023426.g003.jpg

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English: A) HPLC-UV chromatograms of YB and SR extracts with or without PVPP treatment. Asterisk marks internal standard (4-HB). B) After treatment of HUVECs with each 1% tea extract for 20 min, cells were stimulated with thrombin for 5 min and then lysed. Total proteins were analyzed by western blot. Relative band intensity (pMRLC/MRLC) is expressed as a percentage, calculated from value in tea extract-treated cells compared with that in non-treated control cells (+thrombin). Values shown are means ± SEM (n = 3). C) Total ion current chromatograms of SR with or without PVPP treatment. Asterisk marks internal standard (4-HB). D) OPLS-DA score plots and E) loading S-plots derived from each LC-MS data set (SR vs SR+PVPP). S-plot shows the covariance w against the correlation p (corr) of variables of the discriminating component of OPLS-DA model. Cut-off values for the p (corr) < |0.8| were used to select metabolites that most strongly contributed to differences between two tea extracts.
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Source Image file from Fujimura Y, Kurihara K, Ida M, Kosaka R, Miura D, Wariishi H, Maeda-Yamamoto M, Nesumi A, Saito T, Kanda T, Yamada K, Tachibana H (2011). "Metabolomics-Driven Nutraceutical Evaluation of Diverse Green Tea Cultivars". PLOS ONE. DOI:10.1371/journal.pone.0023426. PMID 21853132. PMC: 3154454.
Author Fujimura Y, Kurihara K, Ida M, Kosaka R, Miura D, Wariishi H, Maeda-Yamamoto M, Nesumi A, Saito T, Kanda T, Yamada K, Tachibana H
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