File:Maturation-of-Filopodia-Shaft-Adhesions-Is-Upregulated-by-Local-Cycles-of-Lamellipodia-Advancements-pone.0107097.s013.ogv

English: During the fast spreading of a REF52-β3-integrin-EGFP cell, the fast advancing cell edge could rapidly surpass the filopodia adhesion and leave it behind in the cell lamellum, resulting in its rapid disassembly (at the horizontal arrow, Fig. 6B–b, I). (Fig. 6B). Alternatively, the fast advancing cell edge could abruptly pause for long duration at the filopodia adhesion, resulting in its stable maturation (at the vertical arrow, Fig. 6B–b, II). Overlays of membrane lipid fluorescence (red) and β3-integrin-EGFP (green). 5 min–9 min 36 s after plating the cell on FN coated glass. The time-lapse seqeunce was collected at 12 s/frame with an Olympus FV1000 confocal microscope. The video is shown at 5 frames/s.