File:Mammalian-Diaphanous-Related-Formin-1-Regulates-GSK3β-Dependent-Microtubule-Dynamics-Required-for-T-pone.0080500.s007.ogv
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DescriptionMammalian-Diaphanous-Related-Formin-1-Regulates-GSK3β-Dependent-Microtubule-Dynamics-Required-for-T-pone.0080500.s007.ogv |
English: Representative video showing the invivo interstitial migration of wild-type (green) and mDia1-/- (red) T cells. Naive T cells from mDia1-/- and wild-type mice were labeled with CFSE and CMTMR, respectively and injected 1:1 intravenously into B6 mice. Mice were sacrificed 24 hours after injection and their cervical or axillary lymph nodes imaged with Zeiss LSM 510 META NLO using the FLUAR 20×/0.75 NA objective lens and Zeiss software for image acquisition. For 3-D time lapse imaging, each xy plane spanned 256×256 um at 3um spacing and 60um depth (20 xy planes in each z-stack). Each z stack was imaged at 20 second intervals over a period of 5 minutes. The data are representative of six independent experiments. |
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Source | Video S3 from Dong B, Zhang S, Gao W, Su H, Chen J, Jin F, Bhargava A, Chen X, Jorgensen L, Alberts A, Zhang J, Siminovitch K (2013). "Mammalian Diaphanous-Related Formin 1 Regulates GSK3β-Dependent Microtubule Dynamics Required for T Cell Migratory Polarization". PLOS ONE. DOI:10.1371/journal.pone.0080500. PMID 24260404. PMC: 3832380. | ||
Author | Dong B, Zhang S, Gao W, Su H, Chen J, Jin F, Bhargava A, Chen X, Jorgensen L, Alberts A, Zhang J, Siminovitch K | ||
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This file is licensed under the Creative Commons Attribution 3.0 Unported license.
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current | 03:50, 15 October 2016 | 3.1 s, 1,440 × 1,080 (1.17 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Dong B, Zhang S, Gao W, Su H, Chen J, Jin F, Bhargava A, Chen X, Jorgensen L, Alberts A, Zhang J, Siminovitch K |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Representative video showing the invivo interstitial migration of wild-type (green) and mDia1-/- (red) T cells. Naive T cells from mDia1-/- and wild-type mice were labeled with CFSE and CMTMR, respectively and injected 1:1 intravenously into B6 mice. Mice were sacrificed 24 hours after injection and their cervical or axillary lymph nodes imaged with Zeiss LSM 510 META NLO using the FLUAR 20×/0.75 NA objective lens and Zeiss software for image acquisition. For 3-D time lapse imaging, each xy plane spanned 256×256 um at 3um spacing and 60um depth (20 xy planes in each z-stack). Each z stack was imaged at 20 second intervals over a period of 5 minutes. The data are representative of six independent experiments. |
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Date and time of digitizing | 2013 |