File:Ménage-à-Trois-The-Ratio-of-Bicarbonate-to-CO2-and-the-pH-Regulate-the-Capacity-of-Neutrophils-to-Video 6.ogv

English: The bicarbonate to CO2 ratio influences the neutrophil extracellular trap-inducing potential of monosodium urate crystals (MSU) in RPMI. Neutrophils were cultivated in RPMI containing either 0 mM (top) or 24 mM (bottom) bicarbonate in the copresence of 300 pg/ml MSU. Medium was supplied with 5% CO2. Cells were stained with 5 μM Sytox Green (green) and 0.05 μg/ml Hoechst 33342 (red). Hoechst 33342 (red) stains nuclei of cells with intact plasma membrane, whereas Sytox Green (green) only stains the nuclei of necrotic cells or extracellular DNA. Images were captured every 10 min for 240 min. Microscopy was performed on a BZ-X710 microscope using a time-lapse shooting sequence. Time is indicated in minutes and the scale bar represents 20 μm.