File:Ménage-à-Trois-The-Ratio-of-Bicarbonate-to-CO2-and-the-pH-Regulate-the-Capacity-of-Neutrophils-to-Video 2.ogv

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Original file ‎(Ogg multiplexed audio/video file, Theora/Vorbis, length 4.5 s, 1,756 × 2,304 pixels, 14.54 Mbps overall, file size: 7.87 MB)

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English: High extracellular pH values induce formation of neutrophil extracellular traps in the absence of bicarbonate/CO2 in RPMI. Neutrophils were cultivated in RPMI containing 20 mM HEPES. pH was adjusted either to pH 6.6 (top) or pH 7.8 (bottom). Cells were stained with 5 μM Sytox Green (green). Images were captured every 10 min for 240 min. Microscopy was performed on a BZ-X710 microscope using a time-lapse shooting sequence. Time is indicated in minutes and the scale bar represents 20 μm.
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Source Video S2 from Maueröder C, Mahajan A, Paulus S, Gößwein S, Hahn J, Kienhöfer D, Biermann M, Tripal P, Friedrich R, Munoz L, Neurath M, Becker C, Schett G, Herrmann M, Leppkes M (2016). "Ménage-à-Trois: The Ratio of Bicarbonate to CO2 and the pH Regulate the Capacity of Neutrophils to Form NETs". Frontiers in Immunology. DOI:10.3389/fimmu.2016.00583. PMID 28018350. PMC: 5145884.
Author Maueröder C, Mahajan A, Paulus S, Gößwein S, Hahn J, Kienhöfer D, Biermann M, Tripal P, Friedrich R, Munoz L, Neurath M, Becker C, Schett G, Herrmann M, Leppkes M
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current09:41, 1 February 20174.5 s, 1,756 × 2,304 (7.87 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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