File:Integrin-Dependent-Activation-of-the-JNK-Signaling-Pathway-by-Mechanical-Stress-pone.0026182.s010.ogv

English: Dynamics of S2R+ cells response to mechanical stretch. S2R+ cells transiently transfected with pMT-GFP-Tubulin were plated on collagen-coated silicone membranes and mounted in a Stage Flexer set up. We performed time-lapse imaging of the cells using an upright Leica SPE confocal microscope with a 40× water immersion objective at intervals of 3 minutes. Recording lasted for 45 minutes at resting condition and for a further 60 minutes upon induction of static stretch. Before stretch, S2R+ cells display active cytoskeletal dynamics and stably maintain a stretched morphology. In response to mechanical stress, plated cells withdraw protrusions and retract, rounding up by 60 minutes. In this period, the donor FL shifts (black to red) from 2.43±0.15 ns to 2.18±0.15 ns reflecting an increase in JNK signaling.