File:Induction-of-protein-body-formation-in-plant-leaves-by-elastin-like-polypeptide-fusions-1741-7007-7-48-S8.ogv
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DescriptionInduction-of-protein-body-formation-in-plant-leaves-by-elastin-like-polypeptide-fusions-1741-7007-7-48-S8.ogv |
English: Movie S8. Overexpression of a myosin tail inhibits movement of the novel protein bodies. A dominant-negative mutant of myosin XI-K was co-agro-infiltrated with pPGEK into the leaves of Nicotiana benthamiana and visualized 3 days post-transfection by confocal microscopy. As a result, protein body (PB) trafficking was prevented with the PBs simply oscillating in place, suggesting that a functional actomyosin motility system is required for active PB movement, but is not necessary for the formation of PBs. Fifty image frames were taken over the course of 3 min 34 s. |
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Date | |||
Source | Conley A, Joensuu J, Menassa R, Brandle J (2009). "Induction of protein body formation in plant leaves by elastin-like polypeptide fusions". BMC Biology. DOI:10.1186/1741-7007-7-48. PMID 19664215. PMC: 3224952. | ||
Author | Conley A, Joensuu J, Menassa R, Brandle J | ||
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This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 10:20, 20 November 2012 | 5.0 s, 1,106 × 1,099 (1.36 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 8 |
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Author | Conley A, Joensuu J, Menassa R, Brandle J |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | Movie S8. Overexpression of a myosin tail inhibits movement of the novel protein bodies. A dominant-negative mutant of myosin XI-K was co-agro-infiltrated with pPGEK into the leaves of Nicotiana benthamiana and visualized 3 days post-transfection by confocal microscopy. As a result, protein body (PB) trafficking was prevented with the PBs simply oscillating in place, suggesting that a functional actomyosin motility system is required for active PB movement, but is not necessary for the formation of PBs. Fifty image frames were taken over the course of 3 min 34 s. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2009 |
Categories:
- Microfilaments
- Agrobacterium tumefaciens
- Microscopic images of Nicotiana leaves
- Microscopic videos of Nicotiana benthamiana
- Chloroplasts
- Videos of cytoplasm
- Endoplasmic reticulum
- Extracellular fluid
- Plant gene expression regulation
- Heat-shock proteins
- Ligase chain reaction
- Luminescent agents
- Luminescent proteins
- Videos of peptides
- Polymerase chain reaction
- Protein transport
- Recombinant fusion proteins
- Talin