File:Hypoxia-Transiently-Sequesters-Mps1-and-Polo-to-Collagenase-Sensitive-Filaments-in-Drosophila-pone.0007544.s002.ogv

English: A live GFP-Mps1 oocyte shows GFP localization to filaments in response to hypoxia. This stage 13 GFP-Mps1 oocyte was imaged prior to application of CO2 (Fig. 2A), and then hypoxia was induced by turning on the flow of CO2. The first filaments became visible after approximately eight minutes of hypoxia. Then a 10 µm image stack (Δz = 0.2 µm) was acquired (Fig. 2B). Live imaging began with 2 µm stacks (Δz = 0.4 µm) acquired every 90 seconds. After the first time point (t = 0:00), the flow of gas was turned off, restoring normoxia. This caused the GFP filaments to disperse back into the ooplasm, with no filaments visible by t = 13:30 (Fig. 2C). The gas was turned back on after this time point, and the filaments first became visible again approximately 7:30 later (t = 21:00), and then became progressively brighter through the end of filming at t = 30:00. With the CO2 still on, a 10 µm image stack (Δz = 0.2 µm) was acquired (Fig. 2D). All times are rounded up to the nearest second.