File:High-Throughput-Analysis-of-Stimulus-Evoked-Behaviors-in-Drosophila-Larva-Reveals-Multiple-Modality-pone.0071706.s005.ogv

English: Movie of larval reaction to noxious heat. Movie of contours of larvae in the noxious heat assay obtained with the MWT software. Inside each contour the spine is shown as a white line divided into 10 segments. Short white lines perpendicular to the spine are used to determine the width of the animal. Green triangle marks the onset of a 1 sec long IR laser pulse that induces the noxious heat stimulus. In this experiment the black dots were painted on the back of the animals. Prior to stimulation larvae crawl and occasionally head cast and turn. In response to stimulus onset larvae strongly bend and then roll. Since the black dots are painted on the back of the animal (as shown in Figure 1B), the animals do not have a preferred direction of rolling: some roll to the left, some to the right. Following the roll animals crawl faster than prior to stimulation and we call this behavior escape crawl.