File:High-Refractive-Index-Silicone-Gels-for-Simultaneous-Total-Internal-Reflection-Fluorescence-and-pone.0023807.s003.ogv

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High-Refractive-Index-Silicone-Gels-for-Simultaneous-Total-Internal-Reflection-Fluorescence-and-pone.0023807.s003.ogv ‎(Ogg Theora video file, length 7.4 s, 256 × 256 pixels, 705 kbps, file size: 634 KB)

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English: Real-time movie of a high refractive index gel substrate with a suspension of 100 nm red fluorescent beads (excitation/emission 580/605 fluorescent spheres by Invitrogen, Carlsbad, CA) above the gel taken with TIRF microscopy using a 60x/1.49 objective. Bright objects are either steady dots, which are beads stuck to the gel surface, or less bright dots that disappear immediately after appearing (blinking), as expected for beads entering and exiting (due to their Brownian motion) an ∼100 nm deep region of evanescent illumination above the gel.
Date
Source Movie S1 from Gutierrez E, Tkachenko E, Besser A, Sundd P, Ley K, Danuser G, Ginsberg M, Groisman A (2011). "High Refractive Index Silicone Gels for Simultaneous Total Internal Reflection Fluorescence and Traction Force Microscopy of Adherent Cells". PLOS ONE. DOI:10.1371/journal.pone.0023807. PMID 21961031. PMC: 3178539.
Author Gutierrez E, Tkachenko E, Besser A, Sundd P, Ley K, Danuser G, Ginsberg M, Groisman A
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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Date/TimeThumbnailDimensionsUserComment
current16:34, 14 November 20127.4 s, 256 × 256 (634 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 143 kbps Completed 17:04, 28 August 2018 3.0 s
Streaming 240p (VP9) 143 kbps Completed 00:05, 2 January 2024 1.0 s
WebM 360P 208 kbps Completed 13:15, 30 November 2023 0.0 s
Streaming 144p (MJPEG) 536 kbps Completed 17:01, 14 November 2023 1.0 s

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