File:HAM-5-Functions-As-a-MAP-Kinase-Scaffold-during-Cell-Fusion-in-Neurospora-crassa-pgen.1004783.s012.ogv

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Original file ‎(Ogg Theora video file, length 17 s, 1,024 × 1,024 pixels, 3.03 Mbps, file size: 6.2 MB)

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English: Oscillatory movement of HAM-5-GFP and MAK-2-mCherry to punta, to the hyphal tip and eventual site of contact in hyphae undergoing chemotropic interactions and fusion. A 60-minute movie compressed in 17 seconds of two hyphae undergoing chemotropic interactions and cell fusion. Oscillatory co-recruitment of HAM-5-GFP and MAK-2-mCherry to puncta, to hyphal tips undergoing chemotropic growth, to sites surrounding the septum, and to the site of contact is observed. MAK-2-mCherry is also observed in nuclei, but HAM-5-GFP is not. Alternating oscillation of both HAM-5-GFP and MAK-2-mCherry to puncta and the hyphal tip in the two homing hyphae is observed before fusion, but after fusion, the oscillation of HAM-5-GFP and MAK-2-mCherry to puncta and septa is observed simultaneously in both hyphae. The upper left panel is made of brightfields images, the lower left panel of GFP-fluorescent images, the lower right panel of mCherry-fluorescent images and the upper right panel is of merged GFP and mCherry fluorescent images.
Date
Source Movie S5 from Jonkers W, Leeder A, Ansong C, Wang Y, Yang F, Starr T, Camp D, Smith R, Glass N (2014). "HAM-5 Functions As a MAP Kinase Scaffold during Cell Fusion in Neurospora crassa". PLOS Genetics. DOI:10.1371/journal.pgen.1004783. PMID 25412208. PMC: 4238974.
Author Jonkers W, Leeder A, Ansong C, Wang Y, Yang F, Starr T, Camp D, Smith R, Glass N
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Creative Commons CC-Zero This file is made available under the Creative Commons CC0 1.0 Universal Public Domain Dedication.
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Date/TimeThumbnailDimensionsUserComment
current20:47, 10 December 201417 s, 1,024 × 1,024 (6.2 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 720P 518 kbps Completed 07:20, 28 August 2018 16 s
Streaming 720p (VP9) Not ready Unknown status
VP9 480P 195 kbps Completed 07:20, 28 August 2018 8.0 s
Streaming 480p (VP9) 195 kbps Completed 21:37, 14 March 2024 1.0 s
VP9 360P 81 kbps Completed 07:20, 28 August 2018 7.0 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 33 kbps Completed 07:20, 28 August 2018 4.0 s
Streaming 240p (VP9) 33 kbps Completed 12:48, 22 December 2023 1.0 s
WebM 360P 515 kbps Completed 20:47, 10 December 2014 6.0 s
Streaming 144p (MJPEG) 188 kbps Completed 11:09, 14 November 2023 1.0 s

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