File:Green synthesis of enzyme metal-organic framework composites with high stability in protein denaturing solvents.pdf
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DescriptionGreen synthesis of enzyme metal-organic framework composites with high stability in protein denaturing solvents.pdf |
English: Enzyme/metal-organic framework composites with high stability in protein denaturing solvents were reported in this study.
Encapsulation of enzyme in metal-organic frameworks (MOFs) via co-precipitation process was realized, and the generality of the synthesis was validated by using cytochrome c, horseradish peroxidase, and Candida antarctica lipase B as model enzymes. The stability of encapsulated enzyme was greatly increased after immobilization on MOFs. Remarkably, when exposed to protein denaturing solvents including dimethyl sulfoxide, dimethyl formamide, methanol, and ethanol, the enzyme/MOF composites still preserved almost 100% of activity. In contrast, free enzymes retained no more than 20% of their original activities at the same condition. This study shows the extraordinary protecting efect of MOF shell on increasing enzyme stability at extremely harsh conditions. The enzyme immobilized in MOF exhibited enhanced thermal stability and high tolerance towards protein denaturing organic solvents. |
Date | |
Source | Wu et al. (2017). "Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents". Bioresour. Bioprocess (4): 24. Springer Nature. DOI:10.1186/s40643-017-0154-8. |
Author | Xiaoling Wu, Cheng Yang and Jun Ge |
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Short title | Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents |
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Image title | Bioresources and Bioprocessing, doi:10.1186/s40643-017-0154-8 |
Author | Xiaoling Wu |
Keywords | Biocatalysis,Biomineralization,Immobilization |
Software used | Springer |
Conversion program | Acrobat Distiller 10.1.8 (Windows); modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) |
Encrypted | no |
Page size | 595.276 x 790.866 pts |
Version of PDF format | 1.6 |