File:Giardia-Flagellar-Motility-Is-Not-Directly-Required-to-Maintain-Attachment-to-Surfaces-ppat.1002167.s002.ogv

English: Attachment and detachment of a wild type Giardia trophozoite, TIRFM movie. Attachment is captured after a trophozoite skims along a warmed glass substrate. The ventrolateral flange maintains contact with the substrate while the cell is skimming and may be important in substrate recognition. In this example, the lateral crest also maintains close affinity with the substrate; however, the portion of lateral crest that makes contact during the skimming stage is variable. Once the cell begins to attach, a seal is formed with the lateral crest of the ventral disc. The lateral shield, on either side of the cell body, then presses against the substrate, quickly followed by a depression of the bare area plasma membrane. During detachment, the respective steps occur in reverse order. The bare area cell membrane disappears from view, and the posterior cell body and lateral shield lift up. This motion breaks the seal of the lateral crest and the cell proceeds to skimming using the ventrolateral flange.