File:F-actin-rich-contractile-endothelial-pores-prevent-vascular-leakage-during-leukocyte-diapedesis-ncomms10493-s9.ogv

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F-actin-rich-contractile-endothelial-pores-prevent-vascular-leakage-during-leukocyte-diapedesis-ncomms10493-s9.ogv ‎(Ogg Theora video file, length 8.4 s, 552 × 315 pixels, 1.47 Mbps, file size: 1.48 MB)

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English: Supplementary Movie 8 Spatiotemporal activation of DORA RhoA mutant PKN biosensor upon thrombin treatment in EC. Time-lapse Venus/Cer3 ratio images of DORA RhoA mutant PKN biosensor simultaneously recorded with an epifluorescent microscope showing spatiotemporal RhoA activation after thrombin stimulation (1U per ml) in HUVECs. Calibration bar shows RhoA activation (Red) relative to basal RhoA activity (Blue). Left panel shows Venus/Cer3 ratio images and right panel shows CFP emission only. Images were simultaneously acquired by time-lapse wide-field microscopy (Zeiss Observer Z1) using a 40x 1.3 NA oil immersion objective. Frames were taken every 5 s for 15 minutes.
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Source Video file from Heemskerk N, Schimmel L, Oort C, van Rijssel J, Yin T, Ma B, van Unen J, Pitter B, Huveneers S, Goedhart J, Wu Y, Montanez E, Woodfin A, van Buul J (2016). "F-actin-rich contractile endothelial pores prevent vascular leakage during leukocyte diapedesis through local RhoA signalling". Nature Communications. DOI:10.1038/ncomms10493. PMID 26814335. PMC: 4737874.
Author Heemskerk N, Schimmel L, Oort C, van Rijssel J, Yin T, Ma B, van Unen J, Pitter B, Huveneers S, Goedhart J, Wu Y, Montanez E, Woodfin A, van Buul J
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current13:02, 29 October 20168.4 s, 552 × 315 (1.48 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 240P 236 kbps Completed 09:18, 26 August 2018 9.0 s
Streaming 240p (VP9) 236 kbps Completed 04:43, 21 December 2023 11 s
WebM 360P 342 kbps Completed 09:20, 30 November 2023 2.0 s
Streaming 144p (MJPEG) 904 kbps Completed 20:53, 10 November 2023 1.0 s

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