File:F-actin-rich-contractile-endothelial-pores-prevent-vascular-leakage-during-leukocyte-diapedesis-ncomms10493-s6.ogv

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English: Supplementary Movie 5 F-actin rich endothelial pore structure around transcellular migrating monocytes. 3D representation of confocal Z-stack showing Lifeact-GFP (green) endothelial pores around transcellular migrating monocyte. VE-cadherin (white) distribution at the junction is continuous. DAPI staining shows extravasating monocytes (blue) through the EC cell body. Z-stack images were acquired by confocal laser scanning microscopy (LSM510/Meta; Carl Zeiss Micro-Imaging) using a voxel size of 0.06x0.06x0.48 μm and a 63x 1.4 NA oil immersion objective. Following acquisition, the sequences of Z-stack images were analyzed off-line using Imaris which renders the optical sections into 3D models enabling analysis of leukocyte-endothelial interactions.
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Source Video file from Heemskerk N, Schimmel L, Oort C, van Rijssel J, Yin T, Ma B, van Unen J, Pitter B, Huveneers S, Goedhart J, Wu Y, Montanez E, Woodfin A, van Buul J (2016). "F-actin-rich contractile endothelial pores prevent vascular leakage during leukocyte diapedesis through local RhoA signalling". Nature Communications. DOI:10.1038/ncomms10493. PMID 26814335. PMC: 4737874.
Author Heemskerk N, Schimmel L, Oort C, van Rijssel J, Yin T, Ma B, van Unen J, Pitter B, Huveneers S, Goedhart J, Wu Y, Montanez E, Woodfin A, van Buul J
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current13:01, 29 October 201615 s, 1,024 × 1,024 (17.1 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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