File:F-actin-rich-contractile-endothelial-pores-prevent-vascular-leakage-during-leukocyte-diapedesis-ncomms10493-s4.ogv

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F-actin-rich-contractile-endothelial-pores-prevent-vascular-leakage-during-leukocyte-diapedesis-ncomms10493-s4.ogv ‎(Ogg Theora video file, length 18 s, 800 × 600 pixels, 12.74 Mbps, file size: 27.62 MB)

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English: Supplementary Movie 3 F-actin rich endothelial pore structure around paracellular migrating monocyte. 3D representation of confocal Z-stack showing Lifeact-GFP (green) and Lifeact-mCherry (red) positive endothelial membrane structures that together form a paracellular endothelial pore. The endothelial pore has filopodia-like protrusions at the apical site and a cortical actin-ring at the basolateral site. VE-cadherin (white) distribution at the junction is discontinuous at the site where the monocyte breeches the EC junction. DAPI staining shows extravasating monocyte (blue). Z-stack images were acquired by confocal laser scanning microscopy (LSM510/Meta; Carl Zeiss Micro-Imaging) using a voxel size of 0.06x0.06x0.48 μm and a 63x 1.4 NA oil immersion objective. Following acquisition, the sequences of Z-stack images were analyzed off-line using Imaris which renders the optical sections into 3D models enabling analysis of leukocyte-endothelial interactions.
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Source Video file from Heemskerk N, Schimmel L, Oort C, van Rijssel J, Yin T, Ma B, van Unen J, Pitter B, Huveneers S, Goedhart J, Wu Y, Montanez E, Woodfin A, van Buul J (2016). "F-actin-rich contractile endothelial pores prevent vascular leakage during leukocyte diapedesis through local RhoA signalling". Nature Communications. DOI:10.1038/ncomms10493. PMID 26814335. PMC: 4737874.
Author Heemskerk N, Schimmel L, Oort C, van Rijssel J, Yin T, Ma B, van Unen J, Pitter B, Huveneers S, Goedhart J, Wu Y, Montanez E, Woodfin A, van Buul J
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current13:00, 29 October 201618 s, 800 × 600 (27.62 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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