File:F-actin-rich-contractile-endothelial-pores-prevent-vascular-leakage-during-leukocyte-diapedesis-ncomms10493-s3.ogv

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English: Supplementary Movie 2 Spatiotemporal activation of DORA RhoA mutant PKN biosensor during neutrophil TEM. Time-lapse recordings of the DORA RhoA mutant PKN biosensor showing no change in spatiotemporal RhoA activation during neutrophil extravasation under physiological flow conditions (0.8dyne per cm2). Left panels from top to bottom show, Venus/Cer3 ratio images of the DORA RhoA mutant PKN biosensor, Merge and DIC, respectively. Right panels show zoom of the ROI shown in left panels. Calibration bar shows RhoA activation (Red) relative to basal RhoA activity (Blue). Images were simultaneously acquired by time-lapse wide-field microscopy (Zeiss Observer Z1) using a 40x 1.3 NA oil immersion objective. Frames were taken every 5 s for 9 minutes.
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Source Video file from Heemskerk N, Schimmel L, Oort C, van Rijssel J, Yin T, Ma B, van Unen J, Pitter B, Huveneers S, Goedhart J, Wu Y, Montanez E, Woodfin A, van Buul J (2016). "F-actin-rich contractile endothelial pores prevent vascular leakage during leukocyte diapedesis through local RhoA signalling". Nature Communications. DOI:10.1038/ncomms10493. PMID 26814335. PMC: 4737874.
Author Heemskerk N, Schimmel L, Oort C, van Rijssel J, Yin T, Ma B, van Unen J, Pitter B, Huveneers S, Goedhart J, Wu Y, Montanez E, Woodfin A, van Buul J
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current12:58, 29 October 20168.8 s, 481 × 882 (7.13 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 720P 2.07 Mbps Completed 09:17, 26 August 2018 21 s
Streaming 720p (VP9) 2.07 Mbps Completed 05:33, 22 March 2024 1.0 s
VP9 480P 903 kbps Completed 09:17, 26 August 2018 12 s
Streaming 480p (VP9) 903 kbps Completed 04:04, 7 February 2024 1.0 s
VP9 360P 476 kbps Completed 09:17, 26 August 2018 9.0 s
Streaming 360p (VP9) 477 kbps Completed 07:37, 14 March 2024 1.0 s
VP9 240P 236 kbps Completed 09:17, 26 August 2018 6.0 s
Streaming 240p (VP9) 237 kbps Completed 04:42, 21 December 2023 0.0 s
WebM 360P 469 kbps Completed 13:32, 29 October 2016 11 s
Streaming 144p (MJPEG) 930 kbps Completed 20:51, 10 November 2023 1.0 s

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