File:F-actin-rich-contractile-endothelial-pores-prevent-vascular-leakage-during-leukocyte-diapedesis-ncomms10493-s2.ogv

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English: Supplementary Movie 1 Endothelial RhoA is locally and transiently activated during neutrophil extravasation not during adhesion. Time-lapse recordings of the DORA RhoA biosensor showing spatiotemporal RhoA activation during neutrophil TEM under physiological flow conditions (0.8dyne per cm2). Left panels from top to bottom show, Venus/Cer3 ratio images of the DORA RhoA biosensor, Merge and DIC, respectively. Right panels show zoom of the ROI shown in left panels. Calibration bar shows RhoA activation (Red) relative to basal RhoA activity (Blue). Images were simultaneously acquired by time-lapse wide-field microscopy (Zeiss Observer Z1) using a 40x 1.3 NA oil immersion objective. Frames were taken every 4 s for 6 minutes.
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Source Video file from Heemskerk N, Schimmel L, Oort C, van Rijssel J, Yin T, Ma B, van Unen J, Pitter B, Huveneers S, Goedhart J, Wu Y, Montanez E, Woodfin A, van Buul J (2016). "F-actin-rich contractile endothelial pores prevent vascular leakage during leukocyte diapedesis through local RhoA signalling". Nature Communications. DOI:10.1038/ncomms10493. PMID 26814335. PMC: 4737874.
Author Heemskerk N, Schimmel L, Oort C, van Rijssel J, Yin T, Ma B, van Unen J, Pitter B, Huveneers S, Goedhart J, Wu Y, Montanez E, Woodfin A, van Buul J
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current12:57, 29 October 201618 s, 732 × 1,062 (13.86 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 720P 1.67 Mbps Completed 09:17, 26 August 2018 17 s
Streaming 720p (VP9) 1.67 Mbps Completed 05:32, 22 March 2024 1.0 s
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