File:F-actin-rich-contractile-endothelial-pores-prevent-vascular-leakage-during-leukocyte-diapedesis-ncomms10493-s11.ogv

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F-actin-rich-contractile-endothelial-pores-prevent-vascular-leakage-during-leukocyte-diapedesis-ncomms10493-s11.ogv ‎(Ogg Theora video file, length 10 s, 326 × 150 pixels, 386 kbps, file size: 494 KB)

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English: Supplementary Movie 10 Spatiotemporal activation of DORA RhoA mutant PKN biosensor upon histamine stimulation in EC. Time-lapse Venus/Cer3 ratio images of DORA RhoA mutant PKN biosensor after histamine and subsequent antagonist pyrilamine stimulation in Hela cells expressing the histamine receptor. Calibration bar shows RhoA activation (Red) relative to basal RhoA activity (Blue). Left panel shows Venus/Cer3 ratio images and right panel shows CFP emission only. Images were simultaneously acquired by time-lapse wide-field microscopy (Zeiss Observer Z1) using a 40x 1.3 NA oil immersion objective. Frames were taken every 5 s for 15 minutes.
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Source Video file from Heemskerk N, Schimmel L, Oort C, van Rijssel J, Yin T, Ma B, van Unen J, Pitter B, Huveneers S, Goedhart J, Wu Y, Montanez E, Woodfin A, van Buul J (2016). "F-actin-rich contractile endothelial pores prevent vascular leakage during leukocyte diapedesis through local RhoA signalling". Nature Communications. DOI:10.1038/ncomms10493. PMID 26814335. PMC: 4737874.
Author Heemskerk N, Schimmel L, Oort C, van Rijssel J, Yin T, Ma B, van Unen J, Pitter B, Huveneers S, Goedhart J, Wu Y, Montanez E, Woodfin A, van Buul J
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current13:02, 29 October 201610 s, 326 × 150 (494 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P Not ready Unknown status
Streaming 240p (VP9) 166 kbps Completed 04:42, 21 December 2023 4.0 s
WebM 360P 188 kbps Completed 09:20, 30 November 2023 1.0 s
Streaming 144p (MJPEG) 922 kbps Completed 20:50, 10 November 2023 1.0 s

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