File:Examination-of-actin-and-microtubule-dependent-APC-localisations-in-living-mammalian-cells-1471-2121-7-3-S12.ogv
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Examination-of-actin-and-microtubule-dependent-APC-localisations-in-living-mammalian-cells-1471-2121-7-3-S12.ogv (Ogg Theora video file, length 4.8 s, 224 × 200 pixels, 2.59 Mbps, file size: 1.48 MB)
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DescriptionExamination-of-actin-and-microtubule-dependent-APC-localisations-in-living-mammalian-cells-1471-2121-7-3-S12.ogv |
English: Additional File 12 Live imaging of Cytochalasin D treated COS-7 cell expressing GFP-APC. This movie shows two GFP-APC expressing cells forming a cell-cell contact. GFP-APC is present along the cortex at the site of cell-cell contact and on microtubules near a free edge in the upper cell. Upon addition of Cytochalasin D the cell-cell contact between the cells is weakened and undergoes breakage (black arrow). The cortical GFP-APC puncta are then seen to contract towards the cell vertices in the direction of the white arrow. The microtubule-associated GFP-APC appears unaffected by Cytochalasin D treatment, within the constraints imposed by retraction of the cell edge. Image capture rate was 1 frame/min, movie played back at 20 frames/s. |
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Date | |||
Source | Langford K, Askham J, Lee T, Adams M, Morrison E (2006). "Examination of actin and microtubule dependent APC localisations in living mammalian cells". BMC Cell Biology. DOI:10.1186/1471-2121-7-3. PMID 16423286. PMC: 1386658. | ||
Author | Langford K, Askham J, Lee T, Adams M, Morrison E | ||
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This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 01:15, 21 November 2012 | 4.8 s, 224 × 200 (1.48 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional File 12 |
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Author | Langford K, Askham J, Lee T, Adams M, Morrison E |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | Live imaging of Cytochalasin D treated COS-7 cell expressing GFP-APC. This movie shows two GFP-APC expressing cells forming a cell-cell contact. GFP-APC is present along the cortex at the site of cell-cell contact and on microtubules near a free edge in the upper cell. Upon addition of Cytochalasin D the cell-cell contact between the cells is weakened and undergoes breakage (black arrow). The cortical GFP-APC puncta are then seen to contract towards the cell vertices in the direction of the white arrow. The microtubule-associated GFP-APC appears unaffected by Cytochalasin D treatment, within the constraints imposed by retraction of the cell edge. Image capture rate was 1 frame/min, movie played back at 20 frames/s. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2006 |