File:Eukaryotic-opportunists-dominate-the-deep-subsurface-biosphere-in-South-Africa-ncomms9952-s4.ogv

English: Supplementary Movie 3 Star Diamond + 'vacuum cleaner' at -640 meter. The footage shows use of the 'vacuum cleaner' to suck up biofilm from the same borehole/chamber as in SVIDEO 2. The footage concentrates on sampling in the chamber where biofilm grows attached to the rock face. Most of the biofilm is gone due to closing of the borehole in previous to making the footage. Small pieces (greyish) of biofilm can still be seen swirling in the current. In one piece a nematode (Supplementary figure 15) was identified confirming the biofilm is the residency of at least the nematodes. The tube was easily moved around and could be targeted at the rockface (T+32 seconds). The resolution was 640 x 480 at 25 fps and recorded as an AVI file. Trimming and joining of the footage was done using WinX video converter. All footage came from one and the same sampling session. Per journal instruction the file was converted to a MOV format (H264) and deinterlaced using WinX video converter. For scale, the diameter of the borehole is 4.5 cm.