File:Embryogenesis-of-the-First-Circulating-Endothelial-Cells-pone.0060841.s010.ogv

English: A recording demonstrating ‘rolling’ behavior in the lumens of vitelline artery tributaries A number of endothelial cell nuclei are denoted with arrowheads. Early in the movie a few marked cells display an exceedingly slow rolling behavior compared to the speed of RBC as judged by DIC optics. The Tie1-YFP cells appear to be in contact with the luminal face of the arterial endothelium – reminiscent of leukocyte rolling behavior in mature vascular beds. Eventually the presumptive rolling cells are swept away by blood flow. Other Tie1-YFP cells marked by arrowheads appear to vibrate within a local cell-sized domain before abruptly departing the ROI via circulation. Some marked cells display a combination of the two behaviors. At the end of the recording all cells designated by arrowheads have entered circulation. The compression algorithms used in creating this movie result in a loss of resolution compared to the native image resolution (see Methods). This 2 hr Movie was made using a recording rate of 2.1 frames per minute or 28.6 seconds between frames. Mag bar  = 100 µm.