File:Embryogenesis-of-the-First-Circulating-Endothelial-Cells-pone.0060841.s006.ogv

English: A recording of an epiblastic stage embryo (HH4-) electroporated with a DNA plasmid encoding H2B-GFP. As with the specimen above (Movie S4) the H2B-GFP plasmid was electroporated such that only intra-embryonic mesoderm expresses the GFP, as confirmed by comparing the fluorescence recording with the corresponding DIC recording. The middle panel shows a projection of five (5) Z-axis focal planes at the 22.6 hour time point, while the right panel shows the individual image captured at each focal plane. Notably this recording shows fluorescent cells flowing in the blood designated by arrowheads. The time interval for acquiring each focal plane (Z-step) is approximately 100 msec, which fortuitously corresponded to the transit of a blood-borne cell-of-interest (arrowhead) as it flowed from a head vessel into the sinus venosus. The recording rate is 4.6 fph or 13 min between frames. The compression algorithms used in creating this movie result in a loss of resolution compared to the native image files. Mag bars  = 200 µm.