File:Drosophila-Embryos-as-Model-to-Assess-Cellular-and-Developmental-Toxicity-of-Multi-Walled-Carbon-pone.0088681.s003.ogv

English: MWCNTs are not readily engulfed by phagocytic cells of the immune system. MWCNT/DiI/DMSO (red, arrows) were injected into the syncytial blastoderm and mostly incorporated into the cytoplasm of the newly formed cells. Cells are outlined by GFP tagged microtubules (green, Jupiter-GFP). Movie covers 103 min with a frame taken every 1 min. Bar, 10 µm. Haematocytes, phagocytic and migratory cells of the innate immune system (three arrowheads at beginning of movie), become active about 600 min after injection (720 min after fertilisation), clearing cell fragments and foreign substances by phagocytosis. Uptake of debris involves filopodia, thin cellular extensions which are highly mobile. At the end of the movie, labelled MWCNTs (red, arrow) are located close to a haematocyte. The haematocyte extends a filopodium (arrowhead) but this filopodium although directed towards the MWCNTs does not pull the MWCNT into the cell, showing that extracellular MWCNT are not readily recognized by Haematocytes. In total, 5 embryos were injected and recorded.