File:Direct-single-molecule-measurement-of-TCR-triggering-by-agonist-pMHC-in-living-primary-T-cells-elife00778v002.ogv

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Direct-single-molecule-measurement-of-TCR-triggering-by-agonist-pMHC-in-living-primary-T-cells-elife00778v002.ogv ‎(Ogg Theora video file, length 28 s, 229 × 226 pixels, 344 kbps, file size: 1.16 MB)

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English: Simultaneous observation of ZAP70-EGFP recruitment and pMHC:TCR binding immediately after a living AND T cell lands on the SLB. ZAP70-EGFP membrane recruitment (Video 2) and pMHC:TCR binding (Video 3) occur almost immediately after landing. Radial transport of pMHC:TCR:ZAP70 complexes commences immediately after landing. Data were recorded at 1 frame per second with a 500 ms integration time. These data were analyzed to create the spatial map of pMHC and ZAP70 positions displayed in Figure 4A.

DOI:

https://dx.doi.org/10.7554/eLife.00778.010
Date
Source Video 2. from O'Donoghue G, Pielak R, Smoligovets A, Lin J, Groves J (2013). "Direct single molecule measurement of TCR triggering by agonist pMHC in living primary T cells". eLife. DOI:10.7554/eLife.00778. PMID 23840928. PMC: 3701909.
Author O'Donoghue G, Pielak R, Smoligovets A, Lin J, Groves J
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Date/TimeThumbnailDimensionsUserComment
current12:51, 1 August 201328 s, 229 × 226 (1.16 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P Not ready Unknown status
Streaming 240p (VP9) 148 kbps Completed 22:43, 16 December 2023 5.0 s
WebM 360P 162 kbps Completed 04:55, 30 November 2023 1.0 s
Streaming 144p (MJPEG) 258 kbps Completed 02:07, 18 November 2023 1.0 s

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