File:Direct-single-molecule-measurement-of-TCR-triggering-by-agonist-pMHC-in-living-primary-T-cells-elife00778v001.ogv

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Direct-single-molecule-measurement-of-TCR-triggering-by-agonist-pMHC-in-living-primary-T-cells-elife00778v001.ogv ‎(Ogg Theora video file, length 4.0 s, 228 × 218 pixels, 1.09 Mbps, file size: 526 KB)

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English: A 3 s time-lapse video (189 frames) of agonist pMHC interacting with live 5c.c7 T cells (labeled with MCC-Atto488). A long (500 ms) exposure time allows for unambiguous discrimination between TCR-bound and unbound agonist pMHC in the bilayer. The hardware filtering approach utilized here facilitates particle detection in a relatively dense field of fluorophores, allowing for linking particles between frames even when long (1–10s) time lapses are introduced. This technique also has the advantage of using only one probe, compared to other techniques for detecting ligand binding, such as smFRET, which require two different color probes to detect one molecular binding event. These advantages allow for simultaneous two color single molecule tracking and kinetics measurements.

DOI:

https://dx.doi.org/10.7554/eLife.00778.004
Date
Source Video 1. from O'Donoghue G, Pielak R, Smoligovets A, Lin J, Groves J (2013). "Direct single molecule measurement of TCR triggering by agonist pMHC in living primary T cells". eLife. DOI:10.7554/eLife.00778. PMID 23840928. PMC: 3701909.
Author O'Donoghue G, Pielak R, Smoligovets A, Lin J, Groves J
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Date/TimeThumbnailDimensionsUserComment
current12:51, 1 August 20134.0 s, 228 × 218 (526 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P Not ready Unknown status
Streaming 240p (VP9) 217 kbps Completed 22:43, 16 December 2023 1.0 s
WebM 360P 285 kbps Completed 04:55, 30 November 2023 1.0 s
Streaming 144p (MJPEG) 352 kbps Completed 09:57, 3 November 2023 1.0 s

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