File:Direct-Restriction-of-Virus-Release-and-Incorporation-of-the-Interferon-Induced-Protein-BST-2-into-ppat.1000701.s004.ogv

English: Tomographic reconstructed view of cell surface BST-2 in relation to budding virions. HeLa cells were transfected to express HIV-1 lacking vpu, then stained with antibody to BST-2 and processed for transmission electron microscopy as described in Figure 1, except that 250 nm thick sections were cut and a tilt series of images was obtained for tomography using an FEI Titan electron microscope operated at 300 keV. The initial segment of the movie is the raw tilt sequence, which begins with zero degree tilt, goes to +60 degrees, then to -60 degrees, and ends again at 0 degrees. The tilt sequence transitions into a slice-by-slice back projected volume, moving through 380 computed slices. The final segment of the movie is a maximum intensity projection (MIP) rotated about the Y-axis 720 degrees. The tilt sequence was acquired using FEI automated acquisition software. The slice-by-slice back projected volume was processed with the IMOD software package. MIP visualization and movie production was done using Visage Imaging's Amira software package.