File:Development-of-Cysteine-Free-Fluorescent-Proteins-for-the-Oxidative-Environment-pone.0037551.s004.ogv
Development-of-Cysteine-Free-Fluorescent-Proteins-for-the-Oxidative-Environment-pone.0037551.s004.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 8.9 s, 512 × 512 pixels, 3.62 Mbps overall, file size: 3.85 MB)
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DescriptionDevelopment-of-Cysteine-Free-Fluorescent-Proteins-for-the-Oxidative-Environment-pone.0037551.s004.ogv |
English: Live cell imaging of ss-cfSGFP2-prion fusion protein. COS7 cells transiently expressing ss-cfSGFP2-prion was exposed to 0.5 mM CuSO4 in phosphate-buffered saline at pH 5.0 for 3 min at room temperature and images were continuously recorded at 32°C at 1 frame per min. The z-position of images from an objective lens was set constant using the PerfectFocus function of A1R confocal microscopy. Apparent apoptotic cell death after internalization of large aggregates was observed in some cells (left end). The width and height of images were 81.9 µm. |
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Source | Video S1 from Suzuki T, Arai S, Takeuchi M, Sakurai C, Ebana H, Higashi T, Hashimoto H, Hatsuzawa K, Wada I (2012). "Development of Cysteine-Free Fluorescent Proteins for the Oxidative Environment". PLOS ONE. DOI:10.1371/journal.pone.0037551. PMID 22649538. PMC: 3359384. | ||
Author | Suzuki T, Arai S, Takeuchi M, Sakurai C, Ebana H, Higashi T, Hashimoto H, Hatsuzawa K, Wada I | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 07:37, 16 November 2012 | 8.9 s, 512 × 512 (3.85 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Suzuki T, Arai S, Takeuchi M, Sakurai C, Ebana H, Higashi T, Hashimoto H, Hatsuzawa K, Wada I |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Date and time of digitizing | 2012 |
- Protein chemistry
- Protein folding
- Videos of biomedical engineering
- Molecular cell biology
- Cell anatomy
- Videos of organelles
- Cell physiology
- Cysteine
- Videos of Escherichia coli
- Videos of green fluorescent proteins
- Videos of fluorescence microscopy
- Biological models
- Site-directed mutagenesis
- 3T3 cells
- Videos of plasmids
- Videos of prions
- Protein engineering
- DNA sequence analysis
- Media from PLOS ONE